The Neurite Outgrowth Measurement of Dorsal Root Ganglia Explants Cultured on Estrogen and Schwann Cell-Conditioned Medium by Using Image Analysis
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چکیده
S everal ways of promoting nerve regeneration have been discovered including the use of biochemical substances such as growth factors, hormones and physical factors such as electric currents, and magnetic fields. As first reported by Levi-Montalcini, NGF was identified by promoting growth of neurites from primary cultures of chick explants by using visually scored neurite growth. Recently, the neurite growth of dorsal root ganglion (DRG) explants has been widely used for evaluating the promoting effects of neurotrophic factors on cultures. Counting the neurites directly is considered The Neurite Outgrowth Measurement of Dorsal Root Ganglia Explants Cultured on Estrogen and Schwann Cell-Conditioned Medium by Using Image Analysis
منابع مشابه
Collagen as Adherent Substratum and Inducer of Dorsal Root Ganglia Outgrowth
Neurite outgrowth from dorsal root ganglion (DRG) explants is a method of evaluating neurotrophic activity of growth factors. When complete medium containing collagen was supplemented with nerve growth factor (NGF) DRG outgrowth was observed after 18 h. In the absence of NGF and in the presence of collagen, the DRG outgrowth took place after 72 h. In wells not supplemented with collagen gel in ...
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